WebApr 11, 2024 · RNA extraction was performed using the Qiagen RNeasy Plus kit (74034). cDNAs were synthesised with Maxima First Strand cDNA synthesis kit (Molecular Biology, Thermofisher, K1642) Q-PCR were performed using QuantiNova SYBR Green PCR Kit (Qiagen, 208054). Detection was performed using Rotor-Gene Q Real-time PCR cycler … WebFeb 16, 2024 · RNA concentration was detected with Nanodrop spectrophotometer ND-1000 (ThermoFisher) and 2 µg of RNA were reverse transcribed using Superscript-VILO kit (ThermoFisher) according to the manufacturer’s instructions. qRT-PCR was performed on a 1:100 dilution of the reverse transcription reaction per sample, using the Rotor-Gene Q …
Induction of Robust Immune Responses by CpG-ODN-Loaded …
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Isolation, characterization, proteome, miRNAome, and the …
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